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Detection of SARS-CoV-2 RNA by a Multiplex Reverse-Transcription Loop-Mediated Isothermal Amplification Coupled with Melting Curves Analysis

Loop-mediated isothermal amplification (LAMP) is a method of nucleic acid amplification that is more stable and resistant to DNA amplification inhibitors than conventional PCR. LAMP multiplexing with reverse transcription allows for the single-tube amplification of several RNA fragments, including a...

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Detalles Bibliográficos
Autores principales: Oscorbin, Igor P., Shevelev, Georgiy Yu., Pronyaeva, Ksenia A., Stepanov, Andrey A., Shamovskaya, Darya V., Mishukova, Olga V., Pyshnyi, Dmitrii V., Filipenko, Maksim L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8197939/
https://www.ncbi.nlm.nih.gov/pubmed/34072209
http://dx.doi.org/10.3390/ijms22115743