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In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR

COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5′ and 3′-UTR flanking a region of protein-coding ORFs closely resembling cells’ mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate...

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Autores principales: Barreda-Manso, María Asunción, Nieto-Díaz, Manuel, Soto, Altea, Muñoz-Galdeano, Teresa, Reigada, David, Maza, Rodrigo M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201247/
https://www.ncbi.nlm.nih.gov/pubmed/34198800
http://dx.doi.org/10.3390/ijms22116094
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author Barreda-Manso, María Asunción
Nieto-Díaz, Manuel
Soto, Altea
Muñoz-Galdeano, Teresa
Reigada, David
Maza, Rodrigo M.
author_facet Barreda-Manso, María Asunción
Nieto-Díaz, Manuel
Soto, Altea
Muñoz-Galdeano, Teresa
Reigada, David
Maza, Rodrigo M.
author_sort Barreda-Manso, María Asunción
collection PubMed
description COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5′ and 3′-UTR flanking a region of protein-coding ORFs closely resembling cells’ mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3′-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3′-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository.
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spelling pubmed-82012472021-06-15 In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR Barreda-Manso, María Asunción Nieto-Díaz, Manuel Soto, Altea Muñoz-Galdeano, Teresa Reigada, David Maza, Rodrigo M. Int J Mol Sci Article COVID-19 pandemic is caused by betacoronavirus SARS-CoV-2. The genome of this virus is composed of a single strand of RNA with 5′ and 3′-UTR flanking a region of protein-coding ORFs closely resembling cells’ mRNAs. MicroRNAs are endogenous post-transcriptional regulators that target mRNA to modulate protein expression and mediate cellular functions, including antiviral defense. In the present study, we carried out a bioinformatics screening to search for endogenous human microRNAs targeting the 3′-UTR of SARS-CoV-2. Results from the computational techniques allowed us to identify 10 potential candidates. The capacity of 3 of them, together with hsa-miR-138-5p, to target the SARS-CoV-2 3′-UTR was validated in vitro by gene reporter assays. Available information indicates that two of these microRNAs, namely, hsa-miR-3941 and hsa-miR-138-5p, combine effective targeting of SARS-CoV-2 genome with complementary antiviral or protective effects in the host cells that make them potential candidates for therapeutic treatment of most, if not all, COVID-19 variants known to date. All information obtained while conducting the present analysis is available at Open Science Framework repository. MDPI 2021-06-05 /pmc/articles/PMC8201247/ /pubmed/34198800 http://dx.doi.org/10.3390/ijms22116094 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barreda-Manso, María Asunción
Nieto-Díaz, Manuel
Soto, Altea
Muñoz-Galdeano, Teresa
Reigada, David
Maza, Rodrigo M.
In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title_full In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title_fullStr In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title_full_unstemmed In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title_short In Silico and In Vitro Analyses Validate Human MicroRNAs Targeting the SARS-CoV-2 3′-UTR
title_sort in silico and in vitro analyses validate human micrornas targeting the sars-cov-2 3′-utr
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8201247/
https://www.ncbi.nlm.nih.gov/pubmed/34198800
http://dx.doi.org/10.3390/ijms22116094
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