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ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance

In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states i...

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Detalles Bibliográficos
Autores principales: Lismer, Ariane, Lambrot, Romain, Lafleur, Christine, Dumeaux, Vanessa, Kimmins, Sarah
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202352/
https://www.ncbi.nlm.nih.gov/pubmed/34159325
http://dx.doi.org/10.1016/j.xpro.2021.100602
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author Lismer, Ariane
Lambrot, Romain
Lafleur, Christine
Dumeaux, Vanessa
Kimmins, Sarah
author_facet Lismer, Ariane
Lambrot, Romain
Lafleur, Christine
Dumeaux, Vanessa
Kimmins, Sarah
author_sort Lismer, Ariane
collection PubMed
description In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021).
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spelling pubmed-82023522021-06-21 ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance Lismer, Ariane Lambrot, Romain Lafleur, Christine Dumeaux, Vanessa Kimmins, Sarah STAR Protoc Protocol In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021). Elsevier 2021-06-11 /pmc/articles/PMC8202352/ /pubmed/34159325 http://dx.doi.org/10.1016/j.xpro.2021.100602 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lismer, Ariane
Lambrot, Romain
Lafleur, Christine
Dumeaux, Vanessa
Kimmins, Sarah
ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title_full ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title_fullStr ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title_full_unstemmed ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title_short ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
title_sort chip-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202352/
https://www.ncbi.nlm.nih.gov/pubmed/34159325
http://dx.doi.org/10.1016/j.xpro.2021.100602
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