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ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance
In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states i...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202352/ https://www.ncbi.nlm.nih.gov/pubmed/34159325 http://dx.doi.org/10.1016/j.xpro.2021.100602 |
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author | Lismer, Ariane Lambrot, Romain Lafleur, Christine Dumeaux, Vanessa Kimmins, Sarah |
author_facet | Lismer, Ariane Lambrot, Romain Lafleur, Christine Dumeaux, Vanessa Kimmins, Sarah |
author_sort | Lismer, Ariane |
collection | PubMed |
description | In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021). |
format | Online Article Text |
id | pubmed-8202352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-82023522021-06-21 ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance Lismer, Ariane Lambrot, Romain Lafleur, Christine Dumeaux, Vanessa Kimmins, Sarah STAR Protoc Protocol In the field of epigenetic inheritance, delineating molecular mechanisms implicated in the transfer of paternal environmental conditions to descendants has been elusive. This protocol details how to track sperm chromatin intergenerationally. We describe mouse model design to probe chromatin states in single mouse sperm and techniques to assess pre-implantation embryo chromatin and gene expression. We place emphasis on how to obtain high-quality and quantifiable data sets in sperm and embryos, as well as highlight the limitations of working with low input. For complete details on the use and execution of this protocol, please refer to Lismer et al. (2021). Elsevier 2021-06-11 /pmc/articles/PMC8202352/ /pubmed/34159325 http://dx.doi.org/10.1016/j.xpro.2021.100602 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Lismer, Ariane Lambrot, Romain Lafleur, Christine Dumeaux, Vanessa Kimmins, Sarah ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title | ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title_full | ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title_fullStr | ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title_full_unstemmed | ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title_short | ChIP-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
title_sort | chip-seq protocol for sperm cells and embryos to assess environmental impacts and epigenetic inheritance |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8202352/ https://www.ncbi.nlm.nih.gov/pubmed/34159325 http://dx.doi.org/10.1016/j.xpro.2021.100602 |
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