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Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens

Lacticaseibacillus rhamnosus is a recognized probiotic that is widely used in scientific research and clinical applications. This study found that the Lacticaseibacillus rhamnosus GG (LGG) strain can reduce the adhesion of Escherichia coli (E. coli) to primary chicken intestinal epithelial cells by...

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Autores principales: Guo, Mengjiao, Zhang, Congyue, Zhang, Chengcheng, Zhang, Xiaorong, Wu, Yantao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203825/
https://www.ncbi.nlm.nih.gov/pubmed/34140939
http://dx.doi.org/10.3389/fmicb.2021.664604
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author Guo, Mengjiao
Zhang, Congyue
Zhang, Chengcheng
Zhang, Xiaorong
Wu, Yantao
author_facet Guo, Mengjiao
Zhang, Congyue
Zhang, Chengcheng
Zhang, Xiaorong
Wu, Yantao
author_sort Guo, Mengjiao
collection PubMed
description Lacticaseibacillus rhamnosus is a recognized probiotic that is widely used in scientific research and clinical applications. This study found that the Lacticaseibacillus rhamnosus GG (LGG) strain can reduce the adhesion of Escherichia coli (E. coli) to primary chicken intestinal epithelial cells by 75.7% and inhibit 41.7% of the E. coli that adhere to intestinal epithelial cells. Additionally, LGG showed strong inhibitory ability on the growth of E. coli, Staphylococcus aureus, Salmonella Paratyphi B, and Salmonella Enteritidis in vitro. Furthermore, the influence of LGG on the growth performance, intestinal flora, immunity, and disease resistance of chickens was explored. Chickens fed with LGG exhibited increased average daily weight gain and concentrations of sIgA, IgG, and IgM than did controls. After 21 days of feeding, a diet with LGG increased the diversity of intestinal microbiota and maintained intestinal health. Moreover, LGG promoted immunologic barriers by upregulating cytokines and chemokines via the Toll-like receptor. The major pro-inflammatory factors, including Myd88, NF-κB, Il6, and Il8, were upregulated compared to controls. After being challenged with E. coli, the survival rate of chickens fed with LGG was significantly higher than those in the control group, and decreased numbers of E. coli were detected in the heart and lungs of the LGG group. In summary, oral administration of LGG to chickens could improve growth performance, maintain intestinal homeostasis, and enhance innate immune response and disease resistance.
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spelling pubmed-82038252021-06-16 Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens Guo, Mengjiao Zhang, Congyue Zhang, Chengcheng Zhang, Xiaorong Wu, Yantao Front Microbiol Microbiology Lacticaseibacillus rhamnosus is a recognized probiotic that is widely used in scientific research and clinical applications. This study found that the Lacticaseibacillus rhamnosus GG (LGG) strain can reduce the adhesion of Escherichia coli (E. coli) to primary chicken intestinal epithelial cells by 75.7% and inhibit 41.7% of the E. coli that adhere to intestinal epithelial cells. Additionally, LGG showed strong inhibitory ability on the growth of E. coli, Staphylococcus aureus, Salmonella Paratyphi B, and Salmonella Enteritidis in vitro. Furthermore, the influence of LGG on the growth performance, intestinal flora, immunity, and disease resistance of chickens was explored. Chickens fed with LGG exhibited increased average daily weight gain and concentrations of sIgA, IgG, and IgM than did controls. After 21 days of feeding, a diet with LGG increased the diversity of intestinal microbiota and maintained intestinal health. Moreover, LGG promoted immunologic barriers by upregulating cytokines and chemokines via the Toll-like receptor. The major pro-inflammatory factors, including Myd88, NF-κB, Il6, and Il8, were upregulated compared to controls. After being challenged with E. coli, the survival rate of chickens fed with LGG was significantly higher than those in the control group, and decreased numbers of E. coli were detected in the heart and lungs of the LGG group. In summary, oral administration of LGG to chickens could improve growth performance, maintain intestinal homeostasis, and enhance innate immune response and disease resistance. Frontiers Media S.A. 2021-06-01 /pmc/articles/PMC8203825/ /pubmed/34140939 http://dx.doi.org/10.3389/fmicb.2021.664604 Text en Copyright © 2021 Guo, Zhang, Zhang, Zhang and Wu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Guo, Mengjiao
Zhang, Congyue
Zhang, Chengcheng
Zhang, Xiaorong
Wu, Yantao
Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title_full Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title_fullStr Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title_full_unstemmed Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title_short Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens
title_sort lacticaseibacillus rhamnosus reduces the pathogenicity of escherichia coli in chickens
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8203825/
https://www.ncbi.nlm.nih.gov/pubmed/34140939
http://dx.doi.org/10.3389/fmicb.2021.664604
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