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Highly Efficient and Comprehensive Identification of Ethyl Methanesulfonate-Induced Mutations in Nicotiana tabacum L. by Whole-Genome and Whole-Exome Sequencing

Tobacco (Nicotiana tabacum L.) is a complex allotetraploid species with a large 4.5-Gb genome that carries duplicated gene copies. In this study, we describe the development of a whole-exome sequencing (WES) procedure in tobacco and its application to characterize a test population of ethyl methanes...

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Detalles Bibliográficos
Autores principales: Udagawa, Hisashi, Ichida, Hiroyuki, Takeuchi, Takanori, Abe, Tomoko, Takakura, Yoshimitsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8204250/
https://www.ncbi.nlm.nih.gov/pubmed/34140964
http://dx.doi.org/10.3389/fpls.2021.671598