Simple, Inexpensive RNA Isolation and One‐Step RT‐qPCR Methods for SARS‐CoV‐2 Detection and General Use

The most common method for RNA detection involves reverse transcription followed by quantitative polymerase chain reaction (RT‐qPCR) analysis. Commercial one‐step master mixes—which include both a reverse transcriptase and a thermostable polymerase and thus allow performing both the RT and qPCR step...

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Detalles Bibliográficos
Autores principales: Graham, Thomas G.W., Dugast‐Darzacq, Claire, Dailey, Gina M., Darzacq, Xavier, Tjian, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206771/
https://www.ncbi.nlm.nih.gov/pubmed/33905620
http://dx.doi.org/10.1002/cpz1.130

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8206771/
https://www.ncbi.nlm.nih.gov/pubmed/33905620
http://dx.doi.org/10.1002/cpz1.130

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