A minimal hybridization chain reaction (HCR) system using peptide nucleic acids

The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loo...

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Detalles Bibliográficos
Autores principales: Kim, Ki Tae, Angerani, Simona, Winssinger, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208298/
https://www.ncbi.nlm.nih.gov/pubmed/34194712
http://dx.doi.org/10.1039/d1sc01269j
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author Kim, Ki Tae
Angerani, Simona
Winssinger, Nicolas
author_facet Kim, Ki Tae
Angerani, Simona
Winssinger, Nicolas
author_sort Kim, Ki Tae
collection PubMed
description The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γ-peg-modified PNA was found to be beneficial.
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spelling pubmed-82082982021-06-29 A minimal hybridization chain reaction (HCR) system using peptide nucleic acids Kim, Ki Tae Angerani, Simona Winssinger, Nicolas Chem Sci Chemistry The HCR represents a powerful tool for amplification in DNA-based circuitry and sensing applications, yet requires the use of long DNA sequences to grant hairpin metastability. Here we describe a minimal HCR system based on peptide nucleic acids (PNAs). A system comprising a 5-mer stem and 5-mer loop/toehold hairpins was found to be suitable to achieve rapid amplification. These hairpins were shown to yield >10-fold amplification in 2 h and be suitable for the detection of a cancer biomarker on live cells. The use of γ-peg-modified PNA was found to be beneficial. The Royal Society of Chemistry 2021-05-06 /pmc/articles/PMC8208298/ /pubmed/34194712 http://dx.doi.org/10.1039/d1sc01269j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Kim, Ki Tae
Angerani, Simona
Winssinger, Nicolas
A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title_full A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title_fullStr A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title_full_unstemmed A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title_short A minimal hybridization chain reaction (HCR) system using peptide nucleic acids
title_sort minimal hybridization chain reaction (hcr) system using peptide nucleic acids
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8208298/
https://www.ncbi.nlm.nih.gov/pubmed/34194712
http://dx.doi.org/10.1039/d1sc01269j
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