A reporter system for enriching CRISPR/Cas9 knockout cells in technically challenging settings like patient models

CRISPR/Cas9 represents a valuable tool to determine protein function, but technical hurdles limit its use in challenging settings such as cells unable to grow in vitro like primary leukemia cells and xenografts derived thereof (PDX). To enrich CRISPR/Cas9-edited cells, we improved a dual-reporter sy...

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Detalles Bibliográficos
Autores principales: Liu, Wen-Hsin, Völse, Kerstin, Senft, Daniela, Jeremias, Irmela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209181/
https://www.ncbi.nlm.nih.gov/pubmed/34135367
http://dx.doi.org/10.1038/s41598-021-91760-9

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209181/
https://www.ncbi.nlm.nih.gov/pubmed/34135367
http://dx.doi.org/10.1038/s41598-021-91760-9

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