Protocol for evaluating autophagy using LysoTracker staining in the epithelial follicle stem cells of the Drosophila ovary

We have outlined the approach of visualizing autophagy specifically in the epithelial follicle stem cells of the Drosophila ovary using the LysoTracker dye. The advantage of using this protocol is that it details several techniques, including ovary dissection, immunofluorescence, and western blottin...

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Detalles Bibliográficos
Autores principales: Correa, Iliana, Wang, Melissa, Lee, Eric H., Ruiz-Whalen, Dara M., O’Reilly, Alana M., Singh, Tanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209645/
https://www.ncbi.nlm.nih.gov/pubmed/34169286
http://dx.doi.org/10.1016/j.xpro.2021.100592

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209645/
https://www.ncbi.nlm.nih.gov/pubmed/34169286
http://dx.doi.org/10.1016/j.xpro.2021.100592

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