Protocol for clickable photoaffinity labeling and quantitative chemical proteomics

Here, we describe a protocol for a photoaffinity labeling probe strategy for target deconvolution in live cells. We made a chemical probe by incorporation of a photoreactive group to covalently cross-link with adjacent amino acid residues upon UV irradiation. Click chemistry-based enrichment capture...

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Detalles Bibliográficos
Autores principales: Lee, Wankyu, Huang, Zhen, am Ende, Christopher W., Seneviratne, Uthpala
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209655/
https://www.ncbi.nlm.nih.gov/pubmed/34169287
http://dx.doi.org/10.1016/j.xpro.2021.100593

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209655/
https://www.ncbi.nlm.nih.gov/pubmed/34169287
http://dx.doi.org/10.1016/j.xpro.2021.100593

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