Evaluation of glycolytic rates in human hematopoietic stem/progenitor cells after target gene depletion

This protocol describes how to evaluate cellular metabolic state, including the glycolytic activity, in live CD34(+) hematopoietic stem/progenitor cells (HSPCs) upon depletion of a specific target gene. We detail the procedure of enriching mononuclear cells from human umbilical cord blood samples, i...

Descripción completa

Detalles Bibliográficos
Autores principales: Qing, Ying, Gao, Lei, Han, Li, Su, Rui, Chen, Jianjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209740/
https://www.ncbi.nlm.nih.gov/pubmed/34169295
http://dx.doi.org/10.1016/j.xpro.2021.100603

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209740/
https://www.ncbi.nlm.nih.gov/pubmed/34169295
http://dx.doi.org/10.1016/j.xpro.2021.100603

Ejemplares similares