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Evaluation of glycolytic rates in human hematopoietic stem/progenitor cells after target gene depletion

This protocol describes how to evaluate cellular metabolic state, including the glycolytic activity, in live CD34(+) hematopoietic stem/progenitor cells (HSPCs) upon depletion of a specific target gene. We detail the procedure of enriching mononuclear cells from human umbilical cord blood samples, i...

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Detalles Bibliográficos
Autores principales:	Qing, Ying, Gao, Lei, Han, Li, Su, Rui, Chen, Jianjun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209740/ https://www.ncbi.nlm.nih.gov/pubmed/34169295 http://dx.doi.org/10.1016/j.xpro.2021.100603

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