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Evaluation of glycolytic rates in human hematopoietic stem/progenitor cells after target gene depletion
This protocol describes how to evaluate cellular metabolic state, including the glycolytic activity, in live CD34(+) hematopoietic stem/progenitor cells (HSPCs) upon depletion of a specific target gene. We detail the procedure of enriching mononuclear cells from human umbilical cord blood samples, i...
Autores principales: | Qing, Ying, Gao, Lei, Han, Li, Su, Rui, Chen, Jianjun |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8209740/ https://www.ncbi.nlm.nih.gov/pubmed/34169295 http://dx.doi.org/10.1016/j.xpro.2021.100603 |
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