High-fidelity, efficient, and reversible labeling of endogenous proteins using CRISPR-based designer exon insertion

Precise and efficient insertion of large DNA fragments into somatic cells using gene editing technologies to label or modify endogenous proteins remains challenging. Non-specific insertions/deletions (INDELs) resulting from the non-homologous end joining pathway make the process error-prone. Further...

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Detalles Bibliográficos
Autores principales: Zhong, Haining, Ceballos, Cesar C, Massengill, Crystian I, Muniak, Michael A, Ma, Lei, Qin, Maozhen, Petrie, Stefanie Kaech, Mao, Tianyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211447/
https://www.ncbi.nlm.nih.gov/pubmed/34100715
http://dx.doi.org/10.7554/eLife.64911

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8211447/
https://www.ncbi.nlm.nih.gov/pubmed/34100715
http://dx.doi.org/10.7554/eLife.64911

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