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Preparation of E. coli RNA polymerase transcription elongation complexes by selective photoelution from magnetic beads

In vitro studies of transcription frequently require the preparation of defined elongation complexes. Defined transcription elongation complexes (TECs) are typically prepared by constructing an artificial transcription bubble from synthetic oligonucleotides and RNA polymerase. This approach is optim...

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Detalles Bibliográficos
Autor principal: Strobel, Eric J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212663/
https://www.ncbi.nlm.nih.gov/pubmed/34023383
http://dx.doi.org/10.1016/j.jbc.2021.100812