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Preparation of E. coli RNA polymerase transcription elongation complexes by selective photoelution from magnetic beads
In vitro studies of transcription frequently require the preparation of defined elongation complexes. Defined transcription elongation complexes (TECs) are typically prepared by constructing an artificial transcription bubble from synthetic oligonucleotides and RNA polymerase. This approach is optim...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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American Society for Biochemistry and Molecular Biology
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8212663/ https://www.ncbi.nlm.nih.gov/pubmed/34023383 http://dx.doi.org/10.1016/j.jbc.2021.100812 |