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Analytical and clinical evaluation of a heat shock SARS-CoV-2 detection method without RNA extraction for N and E genes RT-qPCR

BACKGROUND: The COVID-19 pandemic has caused significant supply shortages worldwide for SARS-CoV-2 molecular diagnosis, like RNA extraction kits. OBJECTIVE: The aim of our study was to evaluate the clinical performance and analytical sensitivity of a simple SARS-CoV-2 diagnosis protocol based on hea...

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Detalles Bibliográficos
Autores principales: Bruno, Alfredo, de Mora, Domenica, Freire-Paspuel, Byron, Rodriguez, Angel S., Paredes-Espinosa, Maria Belen, Olmedo, Maritza, Sanchez, Martha, Romero, Jennifer, Paez, Michelle, Gonzalez, Manuel, Orlando, Alberto, Garcia-Bereguiain, Miguel Angel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8215877/
https://www.ncbi.nlm.nih.gov/pubmed/34166793
http://dx.doi.org/10.1016/j.ijid.2021.06.038