Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation

Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal con...

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Detalles Bibliográficos
Autores principales: Greulich, Franziska, Mechtidou, Aikaterini, Horn, Teresa, Uhlenhaut, Nina Henriette
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220248/
https://www.ncbi.nlm.nih.gov/pubmed/34189474
http://dx.doi.org/10.1016/j.xpro.2021.100609

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220248/
https://www.ncbi.nlm.nih.gov/pubmed/34189474
http://dx.doi.org/10.1016/j.xpro.2021.100609

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