Cargando…
Protocol for using heterologous spike-ins to normalize for technical variation in chromatin immunoprecipitation
Quantifying differential genome occupancy by chromatin immunoprecipitation (ChIP) remains challenging due to variation in chromatin fragmentation, immunoprecipitation efficiencies, and intertube variability. In this protocol, we add heterologous spike-ins from Drosophila chromatin as an internal con...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220248/ https://www.ncbi.nlm.nih.gov/pubmed/34189474 http://dx.doi.org/10.1016/j.xpro.2021.100609 |