Cargando…

Acute gene inactivation in the adult mouse liver using the CRISPR-Cas9 technology

Genetic manipulation in mice allows the discovery of gene function and biological mechanisms in vivo. The widely used Cre/LoxP system usually takes months to years especially when starting with the production of floxed alleles of a new gene of interest (GOI). Here, we describe a protocol using the C...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Xiao, Xu, Bo-Lin, Chen, Xiao-Wei
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220398/ https://www.ncbi.nlm.nih.gov/pubmed/34189476 http://dx.doi.org/10.1016/j.xpro.2021.100611

Ejemplares similares

Optimized protocols for efficient gene editing in mouse hepatocytes in vivo using CRISPR-Cas9 technology
por: Chen, Yanhao, et al.
Publicado: (2021)

Optimized protocol for gene editing in adipocytes using CRISPR-Cas9 technology
por: Qiu, Yan, et al.
Publicado: (2021)

CRISPR-Cas9-induced gene knockout in zebrafish
por: Medishetti, Raghavender, et al.
Publicado: (2022)

Generation of mouse hippocampal neural precursor cell lines with CRISPR/Cas9-mediated gene knockouts
por: Zocher, Sara, et al.
Publicado: (2021)

T cell-specific inactivation of mouse CD2 by CRISPR/Cas9
por: Beil-Wagner, Jane, et al.
Publicado: (2016)

Fluorescent tagging of endogenous proteins with CRISPR/Cas9 in primary mouse neural stem cells
por: Morrow, Christopher S., et al.
Publicado: (2021)

Exploiting CRISPR/Cas9 to engineer precise segmental deletions in mouse embryonic stem cells
por: Elango, Rajula, et al.
Publicado: (2022)

Generation of gene-of-interest knockouts in murine organoids using CRISPR-Cas9
por: Huber, Anne, et al.
Publicado: (2023)

Generation of Casp8(FL122/123GG) Mice Using CRISPR-Cas9 Technology
por: Pelletier, Stephane, et al.
Publicado: (2020)

Protocol for biallelic tagging of an endogenous gene using CRISPR-Cas9 in human cells
por: Kong, Nannan, et al.
Publicado: (2023)

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
por: Amorim, João Pedro, et al.
Publicado: (2020)

Protocol for Efficient CRISPR/Cas9/AAV-Mediated Homologous Recombination in Mouse Hematopoietic Stem and Progenitor Cells
por: Tran, Ngoc Tung, et al.
Publicado: (2020)

Generating mutant Aedes aegypti mosquitoes using the CRISPR/Cas9 system
por: Li, Hsing-Han, et al.
Publicado: (2021)

Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi
por: Picchi-Constante, Gisele Fernanda Assine, et al.
Publicado: (2022)

Generation and immunofluorescent validation of gene knockouts in adult human colonic organoids using multi-guide RNA CRISPR-Cas9
por: Chan, Dedrick Kok Hong, et al.
Publicado: (2023)

Application of CRISPR/Cas9 Technology to HBV
por: Lin, Guigao, et al.
Publicado: (2015)

Generation of mouse models carrying B cell restricted single or multiplexed loss-of-function mutations through CRISPR-Cas9 gene editing
por: ten Hacken, Elisa, et al.
Publicado: (2023)

CRISPR-Cas9 Mediated Genome Editing in Bicyclus anynana Butterflies
por: Banerjee, Tirtha Das, et al.
Publicado: (2018)

Protocol for the saturation and multiplexing of genetic variants using CRISPR-Cas9
por: Sahu, Sounak, et al.
Publicado: (2023)

Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
por: Burgio, Gaetan
Publicado: (2018)

CRISPR/Cas9-related technologies in liver diseases: from feasibility to future diversity
por: Xu, Tao, et al.
Publicado: (2020)

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
por: Giuliano, Christopher J., et al.
Publicado: (2019)

Gene Inactivation by CRISPR-Cas9 in Nicotiana tabacum BY-2 Suspension Cells
por: Mercx, Sébastien, et al.
Publicado: (2016)

A small cassette enables conditional gene inactivation by CRISPR/Cas9
por: Guzzardo, Paloma M., et al.
Publicado: (2017)

CRISPR-Cas9 screen in human embryonic stem cells to identify genes required for neural differentiation
por: Qi, Shutao, et al.
Publicado: (2022)

Scalable CRISPR-Cas9 chemical genetic screens in non-transformed human cells
por: Lin, Kevin, et al.
Publicado: (2022)

Transformation and CRISPR-Cas9-mediated homologous recombination in the fungus Rhizopus microsporus
por: Lax, Carlos, et al.
Publicado: (2022)

Differentiation and CRISPR-Cas9-mediated genetic engineering of human intestinal organoids
por: Martinez-Silgado, Adriana, et al.
Publicado: (2022)

Protocols for marker-free gene knock-out and knock-down in Kluyveromyces marxianus using CRISPR/Cas9
por: Rajkumar, Arun S, et al.
Publicado: (2021)

Correction to: Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology
por: Burgio, Gaetan
Publicado: (2018)

Optimized protocol to generate genome-wide inactivated Cas9-expressing murine T cells
por: Laprie-Sentenac, Marguerite, et al.
Publicado: (2022)

Highly efficient gene inactivation by adenoviral CRISPR/Cas9 in human primary cells
por: Voets, Olaf, et al.
Publicado: (2017)

Protocol for the Generation of Human Pluripotent Reporter Cell Lines Using CRISPR/Cas9
por: Zhong, Aaron, et al.
Publicado: (2020)

CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumannii
por: Wang, Yu, et al.
Publicado: (2020)

CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Primary Innate Immune Cells
por: Hildreth, Andrew D., et al.
Publicado: (2020)

Protocol for CRISPR-Cas9 modification of glycosylation in 3D organotypic skin models
por: Marinova, Irina N., et al.
Publicado: (2021)

Heritable CRISPR-Cas9 editing of plant genomes using RNA virus vectors
por: Uranga, Mireia, et al.
Publicado: (2023)

Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans
por: Wang, Peng, et al.
Publicado: (2023)

Edition of TFAM gene by CRISPR/Cas9 technology in bovine model
por: de Oliveira, Vanessa Cristina, et al.
Publicado: (2019)

A protocol for introduction of multiple genetic modifications in Saccharomyces cerevisiae using CRISPR/Cas9
por: Mans, Robert, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_o69u9fovfcui3hlbsjabgs9272