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Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes
Selective identification of newly synthesized proteins is challenging because all proteins, both existing and nascent, have the same amino acid pool and are therefore chemically indistinguishable. L-homopropargylglycine is an amino acid analog of methionine containing an alkyne moiety that can under...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220402/ https://www.ncbi.nlm.nih.gov/pubmed/34189479 http://dx.doi.org/10.1016/j.xpro.2021.100616 |