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Protocol for visualizing newly synthesized proteins in primary mouse hepatocytes

Selective identification of newly synthesized proteins is challenging because all proteins, both existing and nascent, have the same amino acid pool and are therefore chemically indistinguishable. L-homopropargylglycine is an amino acid analog of methionine containing an alkyne moiety that can under...

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Detalles Bibliográficos
Autores principales: Shen, Yuqian, Liu, Wenhua, Zuo, Jian, Han, Junhai, Zhang, Zi Chao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220402/
https://www.ncbi.nlm.nih.gov/pubmed/34189479
http://dx.doi.org/10.1016/j.xpro.2021.100616

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