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Adsorption of Horseradish Peroxidase on Metallic Nanoparticles: Effects on Reactive Oxygen Species Detection Using 2′,7′-Dichlorofluorescin Diacetate

[Image: see text] The fluorescent probe 2′,7′-dichlorofluorescein diacetate (DCFH-DA) together with the enzyme horseradish peroxidase (HRP) is widely used in nanotoxicology to study acellular reactive oxygen species (ROS) production from nanoparticles (NPs). This study examined whether HRP adsorbs o...

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Detalles Bibliográficos
Autores principales: Kessler, Amanda, Hedberg, Jonas, McCarrick, Sarah, Karlsson, Hanna L., Blomberg, Eva, Odnevall, Inger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220500/
https://www.ncbi.nlm.nih.gov/pubmed/33856197
http://dx.doi.org/10.1021/acs.chemrestox.0c00430