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Methyltransferase-directed orthogonal tagging and sequencing of miRNAs and bacterial small RNAs

BACKGROUND: Targeted installation of designer chemical moieties on biopolymers provides an orthogonal means for their visualisation, manipulation and sequence analysis. Although high-throughput RNA sequencing is a widely used method for transcriptome analysis, certain steps, such as 3′ adapter ligat...

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Detalles Bibliográficos
Autores principales: Mickutė, Milda, Kvederavičiūtė, Kotryna, Osipenko, Aleksandr, Mineikaitė, Raminta, Klimašauskas, Saulius, Vilkaitis, Giedrius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8220740/
https://www.ncbi.nlm.nih.gov/pubmed/34158037
http://dx.doi.org/10.1186/s12915-021-01053-w