Extractionless nucleic acid detection: a high capacity solution to COVID-19 testing

We describe an extractionless real-time reverse transcriptase-PCR (rRT-PCR) protocol for SARS-CoV-2 nucleic acid detection using heat as an accurate cost-effective high-capacity solution to COVID-19 testing. We present the effect of temperature, transport media, rRT-PCR mastermixes and gene assays o...

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Detalles Bibliográficos
Autores principales: Baksh, Shairaz, Volodko, Natalia, Soucie, Merle, Geier, Sheena Brandon, Diep, Anthony, Rozak, Kallie, Chan, Tak Yin, Mustapha, Jelili, Lai, Raymond, Estey, Mathew, Verity, Bob, Lee, Mao-Cheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Inc. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222080/
https://www.ncbi.nlm.nih.gov/pubmed/34274751
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222080/
https://www.ncbi.nlm.nih.gov/pubmed/34274751
http://dx.doi.org/10.1016/j.diagmicrobio.2021.115458

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