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Reactive oxygen FIB spin milling enables correlative workflow for 3D super-resolution light microscopy and serial FIB/SEM of cultured cells

Correlative light and electron microscopy (CLEM) is a powerful tool for defining the ultrastructural context of molecularly-labeled biological specimens, particularly when superresolution fluorescence microscopy (SRM) is used for CLEM. Current CLEM, however, is limited by the stark differences in sa...

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Detalles Bibliográficos
Autores principales: Wang, Jing, Randolph, Steven, Wu, Qian, Botman, Aurélien, Schardt, Jenna, Bouchet-Marquis, Cedric, Nan, Xiaolin, Rue, Chad, Straw, Marcus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8222390/
https://www.ncbi.nlm.nih.gov/pubmed/34162977
http://dx.doi.org/10.1038/s41598-021-92608-y