Cargando…

High-precision targeting workflow for volume electron microscopy

Cells are 3D objects. Therefore, volume EM (vEM) is often crucial for correct interpretation of ultrastructural data. Today, scanning EM (SEM) methods such as focused ion beam (FIB)–SEM are frequently used for vEM analyses. While they allow automated data acquisition, precise targeting of volumes of...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ronchi, Paolo, Mizzon, Giulia, Machado, Pedro, D’Imprima, Edoardo, Best, Benedikt T., Cassella, Lucia, Schnorrenberg, Sebastian, Montero, Marta G., Jechlinger, Martin, Ephrussi, Anne, Leptin, Maria, Mahamid, Julia, Schwab, Yannick
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Rockefeller University Press 2021
Materias:	Tools
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225610/ https://www.ncbi.nlm.nih.gov/pubmed/34160561 http://dx.doi.org/10.1083/jcb.202104069

Ejemplares similares

Light and electron microscopy continuum-resolution imaging of 3D cell cultures
por: D’Imprima, Edoardo, et al.
Publicado: (2023)

Targeted volume correlative light and electron microscopy of an environmental marine microorganism
por: Mocaer, Karel, et al.
Publicado: (2023)

Fast and precise targeting of single tumor cells in vivo by multimodal correlative microscopy
por: Karreman, Matthia A., et al.
Publicado: (2016)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM
por: Serra Lleti, José M., et al.
Publicado: (2022)

Deep neural network automated segmentation of cellular structures in volume electron microscopy
por: Gallusser, Benjamin, et al.
Publicado: (2022)

Optogenetic activators of apoptosis, necroptosis, and pyroptosis
por: Shkarina, Kateryna, et al.
Publicado: (2022)

Label-retention expansion microscopy
por: Shi, Xiaoyu, et al.
Publicado: (2021)

Protein denaturation at the air-water interface and how to prevent it
por: D'Imprima, Edoardo, et al.
Publicado: (2019)

Object detection networks and augmented reality for cellular detection in fluorescence microscopy
por: Waithe, Dominic, et al.
Publicado: (2020)

Quantitative correlative microscopy reveals the ultrastructural distribution of endogenous endosomal proteins
por: van der Beek, Jan, et al.
Publicado: (2021)

Analysis of conditional colocalization relationships and hierarchies in three-color microscopy images
por: Vega-Lugo, Jesus, et al.
Publicado: (2022)

Parameter-free molecular super-structures quantification in single-molecule localization microscopy
por: Marenda, Mattia, et al.
Publicado: (2021)

Cytoscape: the network visualization tool for GenomeSpace workflows
por: Demchak, Barry, et al.
Publicado: (2014)

Quality assessment in light microscopy for routine use through simple tools and robust metrics
por: Faklaris, Orestis, et al.
Publicado: (2022)

DeepContact: High-throughput quantification of membrane contact sites based on electron microscopy imaging
por: Liu, Liqing, et al.
Publicado: (2022)

CryoEM structures of membrane pore and prepore complex reveal cytolytic mechanism of Pneumolysin
por: van Pee, Katharina, et al.
Publicado: (2017)

The resolution revolution in cryoEM requires high-quality sample preparation: a rapid pipeline to a high-resolution map of yeast fatty acid synthase
por: Joppe, Mirko, et al.
Publicado: (2020)

CyKEGGParser: tailoring KEGG pathways to fit into systems biology analysis workflows
por: Nersisyan, Lilit, et al.
Publicado: (2014)

Subcellular spatial transcriptomics identifies three mechanistically different classes of localizing RNAs
por: Cassella, Lucia, et al.
Publicado: (2022)

Integrating single cell transcriptomics and volume electron microscopy confirms the presence of pancreatic acinar-like cells in sea urchins
por: Paganos, Periklis, et al.
Publicado: (2022)

Genomic variant annotation workflow for clinical applications
por: Thurnherr, Thomas, et al.
Publicado: (2016)

Increasing workflow development speed and reproducibility with Vectools
por: Weirick, Tyler, et al.
Publicado: (2018)

3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy
por: Russell, Matthew R. G., et al.
Publicado: (2017)

Teaching Microscopy Remotely: Two Engaging Options
por: Lionetti, Kathryn A., et al.
Publicado: (2022)

Imaging nanoscale nuclear structures with expansion microscopy
por: Faulkner, Emma L., et al.
Publicado: (2022)

Multiple Requirements for Rab GTPases in the Development of Drosophila Tracheal Dorsal Branches and Terminal Cells
por: Best, Benedikt T., et al.
Publicado: (2020)

A Bioconductor workflow for processing and analysing spatial proteomics data
por: Breckels, Lisa M., et al.
Publicado: (2018)

Cluster Flow: A user-friendly bioinformatics workflow tool
por: Ewels, Philip, et al.
Publicado: (2017)

Incision Precision: Engaging Students during Dissection Labs
por: Faucette, Azure N.
Publicado: (2023)

A Bioconductor workflow for processing, evaluating, and interpreting expression proteomics data
por: Hutchings, Charlotte, et al.
Publicado: (2023)

Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy
por: de Medeiros, Gustavo, et al.
Publicado: (2020)

Fast4DReg – fast registration of 4D microscopy datasets
por: Pylvänäinen, Joanna W., et al.
Publicado: (2023)

Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast
por: Hinterndorfer, Kerstin, et al.
Publicado: (2022)

TCGA Workflow: Analyze cancer genomics and epigenomics data using Bioconductor packages
por: Silva, Tiago C., et al.
Publicado: (2016)

MetaDEGalaxy: Galaxy workflow for differential abundance analysis of 16s metagenomic data
por: Thang, Mike W.C., et al.
Publicado: (2019)

rANOMALY: AmplicoN wOrkflow for Microbial community AnaLYsis
por: Theil, Sebastien, et al.
Publicado: (2021)

GIANI – open-source software for automated analysis of 3D microscopy images
por: Barry, David J., et al.
Publicado: (2022)

The Diverse World of Protists—an Ideal Community with which to Introduce Microscopy in the Microbiology Teaching Laboratory
por: Van Etten, Julia, et al.
Publicado: (2022)

Cryo-EM structure of the bifunctional secretin complex of Thermus thermophilus
por: D'Imprima, Edoardo, et al.
Publicado: (2017)

The Dockstore: enabling modular, community-focused sharing of Docker-based genomics tools and workflows
por: O'Connor, Brian D., et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_t7dnk7m3lrl0vtbkf5bhniq6qg