Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines

During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluo...

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Detalles Bibliográficos
Autores principales: Xie, Guobin, Zhou, Yuqi, Du, Mingxuan, Wang, Qiqiang, Yi, Jiajin, Zhang, Xiao-kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225967/
https://www.ncbi.nlm.nih.gov/pubmed/34195669
http://dx.doi.org/10.1016/j.xpro.2021.100495

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225967/
https://www.ncbi.nlm.nih.gov/pubmed/34195669
http://dx.doi.org/10.1016/j.xpro.2021.100495

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