Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines

During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluo...

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Autores principales: Xie, Guobin, Zhou, Yuqi, Du, Mingxuan, Wang, Qiqiang, Yi, Jiajin, Zhang, Xiao-kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225967/
https://www.ncbi.nlm.nih.gov/pubmed/34195669
http://dx.doi.org/10.1016/j.xpro.2021.100495
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author Xie, Guobin
Zhou, Yuqi
Du, Mingxuan
Wang, Qiqiang
Yi, Jiajin
Zhang, Xiao-kun
author_facet Xie, Guobin
Zhou, Yuqi
Du, Mingxuan
Wang, Qiqiang
Yi, Jiajin
Zhang, Xiao-kun
author_sort Xie, Guobin
collection PubMed
description During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluorescence, in situ proximity ligation assay, and centrosome isolation. This protocol can be used to identify other transcription factors associated with the centrosome or other subcellular structures during mitotic progression. For complete details on the use and execution of this protocol, please refer to Xie et al. (2020)
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spelling pubmed-82259672021-06-29 Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines Xie, Guobin Zhou, Yuqi Du, Mingxuan Wang, Qiqiang Yi, Jiajin Zhang, Xiao-kun STAR Protoc Protocol During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluorescence, in situ proximity ligation assay, and centrosome isolation. This protocol can be used to identify other transcription factors associated with the centrosome or other subcellular structures during mitotic progression. For complete details on the use and execution of this protocol, please refer to Xie et al. (2020) Elsevier 2021-06-17 /pmc/articles/PMC8225967/ /pubmed/34195669 http://dx.doi.org/10.1016/j.xpro.2021.100495 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Xie, Guobin
Zhou, Yuqi
Du, Mingxuan
Wang, Qiqiang
Yi, Jiajin
Zhang, Xiao-kun
Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title_full Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title_fullStr Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title_full_unstemmed Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title_short Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
title_sort protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225967/
https://www.ncbi.nlm.nih.gov/pubmed/34195669
http://dx.doi.org/10.1016/j.xpro.2021.100495
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