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Protocol to identify centrosome-associated transcription factors during mitosis in mammalian cell lines
During eukaryotic cell mitosis, the nuclear envelope disintegrates and transcription factors are dissociated from condensed chromosomes. Here, we describe a protocol to study centrosomal translocation of nuclear receptor RXRα. We detail procedures for HeLa cell synchronization followed by immunofluo...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8225967/ https://www.ncbi.nlm.nih.gov/pubmed/34195669 http://dx.doi.org/10.1016/j.xpro.2021.100495 |