Anti-EGFR V(HH) Antibody under Thermal Stress Is Better Solubilized with a Lysine than with an Arginine SEP Tag

In this study, we assessed the potential of arginine and lysine solubility-enhancing peptide (SEP) tags to control the solubility of a model protein, anti-EGFR V(HH)-7D12, in a thermally denatured state at a high temperature. We produced V(HH)-7D12 antibodies attached with a C-terminal SEP tag made...

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Detalles Bibliográficos
Autores principales: Kibria, Md. Golam, Fukutani, Akari, Akazawa-Ogawa, Yoko, Hagihara, Yoshihisa, Kuroda, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229009/
https://www.ncbi.nlm.nih.gov/pubmed/34072518
http://dx.doi.org/10.3390/biom11060810

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229009/
https://www.ncbi.nlm.nih.gov/pubmed/34072518
http://dx.doi.org/10.3390/biom11060810

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