Carcass Characteristics, Proximate Composition, Fatty Acid Profile, and Oxidative Stability of Pectoralis major and Flexor cruris medialis Muscle of Broiler Chicken Subjected to with or without Level of Electrical Stunning, Slaughter, and Subsequent Bleeding

SIMPLE SUMMARY: The rapid growth in the global and Muslim population has increased the demand for ritually obtained meat, creating a scope of the global meat business. Pre- and post-slaughter practices are of concern in the global meat industries. Therefore, this study examined four types of slaughter, applied with or without a level of electrical stunning, halal neck cutting, and subsequent bleeding (LSHS, MSHS, and HSHS vs. NSHS). Treatments were as follows: (1) NSHS (without electrical stunning, halal neck cut, and subsequent bleeding was for 180 s), (2) LSHS (electrically stunned at 250 mA for 5 s, halal neck cut, and subsequent bleeding was for 180 s), (3) MSHS (electrically stunned at 500 mA for 10 s, halal neck cut, and subsequent bleeding was for 180 s), and (4) HSHS (electrically stunned at 1000 mA for 20 s, halal neck cut, and subsequent bleeding was for 180 s). Slaughtering practices were evaluated in broiler chickens to determine if they influence the carcass characteristics, livability, bleeding out, Pectoralis major and Flexor cruris medialis proximate composition, cholesterol content, fatty acid profile, post-mortem pH, microbial loads, and oxidative stability. With or without stunning, halal neck cutting and subsequent bleeding did not have a significant negative impact on the nutritional aspects, such as proximate composition, cholesterol content, and fatty acid profile, or post-mortem pH, microbial loads except for a variation in some carcass characteristics, livability, bleeding out, and oxidative stability during post-mortem. Since the pre-slaughter conscious state of the animal/bird and post-slaughter bleeding are ritual demands in this process, higher livability and higher bleed out was exhibited in NSHS and LSHS, and there was no large negative impact on nutritional aspects. Therefore, the meat industry can consider without stunning (NSHS) or short-time electrical stunning (LSHS) to capture the global meat market. ABSTRACT: With an emphasis on the global meat market and considering the ritual requirements and quality aspects, four types of slaughtering treatments were compared: (1) NSHS (without electrical stunning, halal neck cut, and subsequent bleeding for 180 s), (2) LSHS (electrically stunned at 250 mA for five seconds, halal neck cut, and subsequent bleeding for 180 s), (3) MSHS (electrically stunned at 500 mA for 10 s, halal neck cut, and subsequent bleeding for 180 s), and (4) HSHS (electrically stunned at 1000 mA for 20 s, halal neck cut, and subsequent bleeding for 180 s). Four hundred 36 day-old Ross 308 broiler chickens (body weights of 1.4 to 1.8 kg) were divided into four random groups of 100 birds each (ten replicated pens of ten birds). This study examined the livability, bleeding out, Pectoralis major and Flexor cruris medialis proximate composition, cholesterol content, fatty acid profile and post-mortem pH, microbial loads, and oxidative stability. The livability and bleeding out were higher in NSHS and LSHS than MSHS and HSHS (p < 0.05). The Pectoralis major and Flexor cruris medialis proximate composition, cholesterol content and fatty acid profile, post-mortem pH, and microbial loads were unaffected by the slaughter treatments (p > 0.05), but the oxidative stability of Pectoralis major differed during the eight-day post-mortem period (p < 0.05). The results suggest that for capturing the global meat market, the meat industry can consider NSHS and LSHS because the ritual requirements are fulfilled, and there is no negative impact on the nutritional aspects.