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Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A

Manumycin A (MA) is a well-tolerated natural antibiotic showing pleiotropic anticancer effects in various preclinical in vitro and in vivo models. Anticancer drugs may themselves act as stressors to induce the cellular adaptive mechanism that can minimize their cytotoxicity. Heat shock proteins (HSP...

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Autores principales: Sojka, Damian Robert, Hasterok, Sylwia, Vydra, Natalia, Toma-Jonik, Agnieszka, Wieczorek, Anna, Gogler-Pigłowska, Agnieszka, Scieglinska, Dorota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229576/
https://www.ncbi.nlm.nih.gov/pubmed/34200371
http://dx.doi.org/10.3390/cells10061418
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author Sojka, Damian Robert
Hasterok, Sylwia
Vydra, Natalia
Toma-Jonik, Agnieszka
Wieczorek, Anna
Gogler-Pigłowska, Agnieszka
Scieglinska, Dorota
author_facet Sojka, Damian Robert
Hasterok, Sylwia
Vydra, Natalia
Toma-Jonik, Agnieszka
Wieczorek, Anna
Gogler-Pigłowska, Agnieszka
Scieglinska, Dorota
author_sort Sojka, Damian Robert
collection PubMed
description Manumycin A (MA) is a well-tolerated natural antibiotic showing pleiotropic anticancer effects in various preclinical in vitro and in vivo models. Anticancer drugs may themselves act as stressors to induce the cellular adaptive mechanism that can minimize their cytotoxicity. Heat shock proteins (HSPs) as cytoprotective factors can counteract the deleterious effects of various stressful stimuli. In this study, we examined whether the anticancer effects of MA can be counteracted by the mechanism related to HSPs belonging to the HSPA (HSP70) family. We found that MA caused cell type-specific alterations in the levels of HSPAs. These changes included concomitant upregulation of the stress-inducible (HSPA1 and HSPA6) and downregulation of the non-stress-inducible (HSPA2) paralogs. However, neither HSPA1 nor HSPA2 were necessary to provide protection against MA in lung cancer cells. Conversely, the simultaneous repression of several HSPA paralogs using pan-HSPA inhibitors (VER-155008 or JG-98) sensitized cancer cells to MA. We also observed that genetic ablation of the heat shock factor 1 (HSF1) transcription factor, a main transactivator of HSPAs expression, sensitized MCF7 cells to MA treatment. Our study reveals that inhibition of HSF1-mediated heat shock response (HSR) can improve the anticancer effect of MA. These observations suggest that targeting the HSR- or HSPA-mediated adaptive mechanisms may be a promising strategy for further preclinical developments.
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spelling pubmed-82295762021-06-26 Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A Sojka, Damian Robert Hasterok, Sylwia Vydra, Natalia Toma-Jonik, Agnieszka Wieczorek, Anna Gogler-Pigłowska, Agnieszka Scieglinska, Dorota Cells Article Manumycin A (MA) is a well-tolerated natural antibiotic showing pleiotropic anticancer effects in various preclinical in vitro and in vivo models. Anticancer drugs may themselves act as stressors to induce the cellular adaptive mechanism that can minimize their cytotoxicity. Heat shock proteins (HSPs) as cytoprotective factors can counteract the deleterious effects of various stressful stimuli. In this study, we examined whether the anticancer effects of MA can be counteracted by the mechanism related to HSPs belonging to the HSPA (HSP70) family. We found that MA caused cell type-specific alterations in the levels of HSPAs. These changes included concomitant upregulation of the stress-inducible (HSPA1 and HSPA6) and downregulation of the non-stress-inducible (HSPA2) paralogs. However, neither HSPA1 nor HSPA2 were necessary to provide protection against MA in lung cancer cells. Conversely, the simultaneous repression of several HSPA paralogs using pan-HSPA inhibitors (VER-155008 or JG-98) sensitized cancer cells to MA. We also observed that genetic ablation of the heat shock factor 1 (HSF1) transcription factor, a main transactivator of HSPAs expression, sensitized MCF7 cells to MA treatment. Our study reveals that inhibition of HSF1-mediated heat shock response (HSR) can improve the anticancer effect of MA. These observations suggest that targeting the HSR- or HSPA-mediated adaptive mechanisms may be a promising strategy for further preclinical developments. MDPI 2021-06-07 /pmc/articles/PMC8229576/ /pubmed/34200371 http://dx.doi.org/10.3390/cells10061418 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sojka, Damian Robert
Hasterok, Sylwia
Vydra, Natalia
Toma-Jonik, Agnieszka
Wieczorek, Anna
Gogler-Pigłowska, Agnieszka
Scieglinska, Dorota
Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title_full Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title_fullStr Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title_full_unstemmed Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title_short Inhibition of the Heat Shock Protein A (HSPA) Family Potentiates the Anticancer Effects of Manumycin A
title_sort inhibition of the heat shock protein a (hspa) family potentiates the anticancer effects of manumycin a
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229576/
https://www.ncbi.nlm.nih.gov/pubmed/34200371
http://dx.doi.org/10.3390/cells10061418
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