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Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes

The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that sub...

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Detalles Bibliográficos
Autores principales: Sibler, Eliane, He, Yuliang, Ducoli, Luca, Keller, Nadja, Fujimoto, Noriki, Dieterich, Lothar C., Detmar, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229892/
https://www.ncbi.nlm.nih.gov/pubmed/34199492
http://dx.doi.org/10.3390/cells10061371
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author Sibler, Eliane
He, Yuliang
Ducoli, Luca
Keller, Nadja
Fujimoto, Noriki
Dieterich, Lothar C.
Detmar, Michael
author_facet Sibler, Eliane
He, Yuliang
Ducoli, Luca
Keller, Nadja
Fujimoto, Noriki
Dieterich, Lothar C.
Detmar, Michael
author_sort Sibler, Eliane
collection PubMed
description The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that subsets of LECs lining the different LN sinuses responded individually to skin inflammation, suggesting that they exert distinct functions under pathological conditions. Among the genes dysregulated during inflammation, we confirmed an up-regulation of CD200 in the LECs lining the subcapsular sinus floor with a possible function in immune regulation. Furthermore, by in silico analysis, we predicted numerous possible interactions of LECs with diverse immune cells in the LNs and found similarities in the transcriptional changes of LN LECs in different skin inflammation settings. In summary, we provide an in-depth analysis of the transcriptional landscape of LN LECs in the naïve state and in skin inflammation.
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spelling pubmed-82298922021-06-26 Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes Sibler, Eliane He, Yuliang Ducoli, Luca Keller, Nadja Fujimoto, Noriki Dieterich, Lothar C. Detmar, Michael Cells Article The lymphatic system plays a crucial role in immunity and lymph nodes (LNs) undergo drastic remodeling during inflammation. Here, we used single-cell RNA sequencing to investigate transcriptional changes in lymphatic endothelial cells (LECs) in LNs draining naïve and inflamed skin. We found that subsets of LECs lining the different LN sinuses responded individually to skin inflammation, suggesting that they exert distinct functions under pathological conditions. Among the genes dysregulated during inflammation, we confirmed an up-regulation of CD200 in the LECs lining the subcapsular sinus floor with a possible function in immune regulation. Furthermore, by in silico analysis, we predicted numerous possible interactions of LECs with diverse immune cells in the LNs and found similarities in the transcriptional changes of LN LECs in different skin inflammation settings. In summary, we provide an in-depth analysis of the transcriptional landscape of LN LECs in the naïve state and in skin inflammation. MDPI 2021-06-02 /pmc/articles/PMC8229892/ /pubmed/34199492 http://dx.doi.org/10.3390/cells10061371 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sibler, Eliane
He, Yuliang
Ducoli, Luca
Keller, Nadja
Fujimoto, Noriki
Dieterich, Lothar C.
Detmar, Michael
Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title_full Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title_fullStr Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title_full_unstemmed Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title_short Single-Cell Transcriptional Heterogeneity of Lymphatic Endothelial Cells in Normal and Inflamed Murine Lymph Nodes
title_sort single-cell transcriptional heterogeneity of lymphatic endothelial cells in normal and inflamed murine lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229892/
https://www.ncbi.nlm.nih.gov/pubmed/34199492
http://dx.doi.org/10.3390/cells10061371
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