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NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses

In the present study, we analyze the nuclear magnetic resonance (NMR) blood serum metabolic profiles of 106 head and neck squamous cell carcinoma (HNSCC) patients during radio (RT) and concurrent radio-chemotherapy (CHRT). Four different fractionation schemes were compared. The blood samples were co...

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Autores principales: Boguszewicz, Łukasz, Bieleń, Agata, Ciszek, Mateusz, Wendykier, Jacek, Szczepanik, Krzysztof, Skorupa, Agnieszka, Mrochem-Kwarciak, Jolanta, Składowski, Krzysztof, Sokół, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231285/
https://www.ncbi.nlm.nih.gov/pubmed/34208417
http://dx.doi.org/10.3390/ijms22126310
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author Boguszewicz, Łukasz
Bieleń, Agata
Ciszek, Mateusz
Wendykier, Jacek
Szczepanik, Krzysztof
Skorupa, Agnieszka
Mrochem-Kwarciak, Jolanta
Składowski, Krzysztof
Sokół, Maria
author_facet Boguszewicz, Łukasz
Bieleń, Agata
Ciszek, Mateusz
Wendykier, Jacek
Szczepanik, Krzysztof
Skorupa, Agnieszka
Mrochem-Kwarciak, Jolanta
Składowski, Krzysztof
Sokół, Maria
author_sort Boguszewicz, Łukasz
collection PubMed
description In the present study, we analyze the nuclear magnetic resonance (NMR) blood serum metabolic profiles of 106 head and neck squamous cell carcinoma (HNSCC) patients during radio (RT) and concurrent radio-chemotherapy (CHRT). Four different fractionation schemes were compared. The blood samples were collected weekly, from the day before the treatment until the last week of CHRT/RT. The NMR spectra were acquired on A Bruker 400 MHz spectrometer at 310 K and analyzed using multivariate methods. Seven metabolites were found significantly to be altered solely by radiotherapy: N-acetyl-glycoprotein (NAG), N-acetylcysteine, glycerol, glycolate and the lipids at 0.9, 1.3 and 3.2 ppm. The NMR results were correlated with the tissue volumes receiving a particular dose of radiation. The influence of the irradiated volume on the metabolic profile is weak and mainly limited to sparse correlations with the inflammatory markers, creatinine and the lymphocyte count in RT and the branched-chain amino-acids in CHRT. This is probably due to the optimal planning and delivery of radiotherapy improving sparing of the surrounding normal tissues and minimizing the differences between the patients (caused by the tumor location and size).
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spelling pubmed-82312852021-06-26 NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses Boguszewicz, Łukasz Bieleń, Agata Ciszek, Mateusz Wendykier, Jacek Szczepanik, Krzysztof Skorupa, Agnieszka Mrochem-Kwarciak, Jolanta Składowski, Krzysztof Sokół, Maria Int J Mol Sci Article In the present study, we analyze the nuclear magnetic resonance (NMR) blood serum metabolic profiles of 106 head and neck squamous cell carcinoma (HNSCC) patients during radio (RT) and concurrent radio-chemotherapy (CHRT). Four different fractionation schemes were compared. The blood samples were collected weekly, from the day before the treatment until the last week of CHRT/RT. The NMR spectra were acquired on A Bruker 400 MHz spectrometer at 310 K and analyzed using multivariate methods. Seven metabolites were found significantly to be altered solely by radiotherapy: N-acetyl-glycoprotein (NAG), N-acetylcysteine, glycerol, glycolate and the lipids at 0.9, 1.3 and 3.2 ppm. The NMR results were correlated with the tissue volumes receiving a particular dose of radiation. The influence of the irradiated volume on the metabolic profile is weak and mainly limited to sparse correlations with the inflammatory markers, creatinine and the lymphocyte count in RT and the branched-chain amino-acids in CHRT. This is probably due to the optimal planning and delivery of radiotherapy improving sparing of the surrounding normal tissues and minimizing the differences between the patients (caused by the tumor location and size). MDPI 2021-06-11 /pmc/articles/PMC8231285/ /pubmed/34208417 http://dx.doi.org/10.3390/ijms22126310 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Boguszewicz, Łukasz
Bieleń, Agata
Ciszek, Mateusz
Wendykier, Jacek
Szczepanik, Krzysztof
Skorupa, Agnieszka
Mrochem-Kwarciak, Jolanta
Składowski, Krzysztof
Sokół, Maria
NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title_full NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title_fullStr NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title_full_unstemmed NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title_short NMR-Based Metabolomics in Investigation of the Radiation Induced Changes in Blood Serum of Head and Neck Cancer Patients and Its Correlation with the Tissue Volumes Exposed to the Particulate Doses
title_sort nmr-based metabolomics in investigation of the radiation induced changes in blood serum of head and neck cancer patients and its correlation with the tissue volumes exposed to the particulate doses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231285/
https://www.ncbi.nlm.nih.gov/pubmed/34208417
http://dx.doi.org/10.3390/ijms22126310
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