CRISPR-Mediated Endogenous Activation of Fibroin Heavy Chain Gene Triggers Cellular Stress Responses in Bombyx mori Embryonic Cells

SIMPLE SUMMARY: Based on a CRISPRa approach, activating endogenous fibroin heavy chain (FibH) gene expression in Bombyx mori embryonic (BmE) cells, which was driven by a combination of the dCas9-VPR (a tripartite activator, composed of VP64, p65, and Rta) and the sgRNA targeting to the promoter of F...

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Detalles Bibliográficos
Autores principales: Hu, Wenbo, Wang, Xiaogang, Ma, Sanyuan, Peng, Zhangchuan, Cao, Yang, Xia, Qingyou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231919/
https://www.ncbi.nlm.nih.gov/pubmed/34199296
http://dx.doi.org/10.3390/insects12060552

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8231919/
https://www.ncbi.nlm.nih.gov/pubmed/34199296
http://dx.doi.org/10.3390/insects12060552

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