Preparation of the intact rodent organ of Corti for RNAscope and immunolabeling, confocal microscopy, and quantitative analysis

This protocol describes the preparation of the mouse organ of Corti for RNAscope, immunolabeling, confocal microscopy, and quantitative image analysis to examine transcript and protein localization, sensory hair cells, and synapses. This protocol can be applied to mice and other rodents (juvenile an...

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Detalles Bibliográficos
Autores principales: Reijntjes, Daniel O.J., Breitzler, J. Lukas, Persic, Dora, Pyott, Sonja J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233256/
https://www.ncbi.nlm.nih.gov/pubmed/34195667
http://dx.doi.org/10.1016/j.xpro.2021.100544

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8233256/
https://www.ncbi.nlm.nih.gov/pubmed/34195667
http://dx.doi.org/10.1016/j.xpro.2021.100544

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