Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use

[Image: see text] This work demonstrates the application of hyaluronan-conjugated nitrogen-doped carbon quantum dots (HA-nCQDs) for bioimaging of tumor cells and illustrates their potential use as carriers in targeted drug delivery. Quantum dots are challenging to deliver with specificity, which hin...

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Autores principales: Karakoçak, Bedia Begüm, Laradji, Amine, Primeau, Tina, Berezin, Mikhail Y., Li, Shunqiang, Ravi, Nathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243741/
https://www.ncbi.nlm.nih.gov/pubmed/33355448
http://dx.doi.org/10.1021/acsami.0c20088
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author Karakoçak, Bedia Begüm
Laradji, Amine
Primeau, Tina
Berezin, Mikhail Y.
Li, Shunqiang
Ravi, Nathan
author_facet Karakoçak, Bedia Begüm
Laradji, Amine
Primeau, Tina
Berezin, Mikhail Y.
Li, Shunqiang
Ravi, Nathan
author_sort Karakoçak, Bedia Begüm
collection PubMed
description [Image: see text] This work demonstrates the application of hyaluronan-conjugated nitrogen-doped carbon quantum dots (HA-nCQDs) for bioimaging of tumor cells and illustrates their potential use as carriers in targeted drug delivery. Quantum dots are challenging to deliver with specificity, which hinders their application. To facilitate targeted internalization by cancer cells, hyaluronic acid, a natural ligand of CD44 receptors, was covalently grafted on nCQDs. The HA-nCQD conjugate was synthesized by carbodiimide coupling of the amine moieties on nCQDs and the carboxylic acids on HA chains. Conjugated HA-nCQD retained sufficient fluorescence, although with 30% lower quantum efficiency than the original nCQDs. Confocal microscopy showed enhanced internalization of HA-nCQDs, facilitated by CD44 receptors. To demonstrate the specificity of HA-nCQDs toward human tumor cells, patient-derived breast cancer tissue with high-CD44 expression was implanted in adult mice. The tumors were allowed to grow up to 200–250 mm(3) prior to the injection of HA-nCQDs. With either local or systemic injection, we achieved a high level of tumor specificity judged by a strong signal-to-noise ratio between the tumor and the surrounding tissue in vivo. Overall, the results show that HA-nCQDs can be used for imaging of CD44-specific tumors in preclinical models of human cancer and potentially used as carriers for targeted drug delivery into CD44-rich cells.
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spelling pubmed-82437412022-01-13 Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use Karakoçak, Bedia Begüm Laradji, Amine Primeau, Tina Berezin, Mikhail Y. Li, Shunqiang Ravi, Nathan ACS Appl Mater Interfaces [Image: see text] This work demonstrates the application of hyaluronan-conjugated nitrogen-doped carbon quantum dots (HA-nCQDs) for bioimaging of tumor cells and illustrates their potential use as carriers in targeted drug delivery. Quantum dots are challenging to deliver with specificity, which hinders their application. To facilitate targeted internalization by cancer cells, hyaluronic acid, a natural ligand of CD44 receptors, was covalently grafted on nCQDs. The HA-nCQD conjugate was synthesized by carbodiimide coupling of the amine moieties on nCQDs and the carboxylic acids on HA chains. Conjugated HA-nCQD retained sufficient fluorescence, although with 30% lower quantum efficiency than the original nCQDs. Confocal microscopy showed enhanced internalization of HA-nCQDs, facilitated by CD44 receptors. To demonstrate the specificity of HA-nCQDs toward human tumor cells, patient-derived breast cancer tissue with high-CD44 expression was implanted in adult mice. The tumors were allowed to grow up to 200–250 mm(3) prior to the injection of HA-nCQDs. With either local or systemic injection, we achieved a high level of tumor specificity judged by a strong signal-to-noise ratio between the tumor and the surrounding tissue in vivo. Overall, the results show that HA-nCQDs can be used for imaging of CD44-specific tumors in preclinical models of human cancer and potentially used as carriers for targeted drug delivery into CD44-rich cells. American Chemical Society 2020-12-23 2021-01-13 /pmc/articles/PMC8243741/ /pubmed/33355448 http://dx.doi.org/10.1021/acsami.0c20088 Text en © 2020 American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Karakoçak, Bedia Begüm
Laradji, Amine
Primeau, Tina
Berezin, Mikhail Y.
Li, Shunqiang
Ravi, Nathan
Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title_full Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title_fullStr Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title_full_unstemmed Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title_short Hyaluronan-Conjugated Carbon Quantum Dots for Bioimaging Use
title_sort hyaluronan-conjugated carbon quantum dots for bioimaging use
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8243741/
https://www.ncbi.nlm.nih.gov/pubmed/33355448
http://dx.doi.org/10.1021/acsami.0c20088
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