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Aggregation is a Context-Dependent Constraint on Protein Evolution

Solubility is a requirement for many cellular processes. Loss of solubility and aggregation can lead to the partial or complete abrogation of protein function. Thus, understanding the relationship between protein evolution and aggregation is an important goal. Here, we analysed two deep mutational s...

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Autores principales: Monti, Michele, Armaos, Alexandros, Fantini, Marco, Pastore, Annalisa, Tartaglia, Gian Gaetano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249573/
https://www.ncbi.nlm.nih.gov/pubmed/34222334
http://dx.doi.org/10.3389/fmolb.2021.678115
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author Monti, Michele
Armaos, Alexandros
Fantini, Marco
Pastore, Annalisa
Tartaglia, Gian Gaetano
author_facet Monti, Michele
Armaos, Alexandros
Fantini, Marco
Pastore, Annalisa
Tartaglia, Gian Gaetano
author_sort Monti, Michele
collection PubMed
description Solubility is a requirement for many cellular processes. Loss of solubility and aggregation can lead to the partial or complete abrogation of protein function. Thus, understanding the relationship between protein evolution and aggregation is an important goal. Here, we analysed two deep mutational scanning experiments to investigate the role of protein aggregation in molecular evolution. In one data set, mutants of a protein involved in RNA biogenesis and processing, human TAR DNA binding protein 43 (TDP-43), were expressed in S. cerevisiae. In the other data set, mutants of a bacterial enzyme that controls resistance to penicillins and cephalosporins, TEM-1 beta-lactamase, were expressed in E. coli under the selective pressure of an antibiotic treatment. We found that aggregation differentiates the effects of mutations in the two different cellular contexts. Specifically, aggregation was found to be associated with increased cell fitness in the case of TDP-43 mutations, as it protects the host from aberrant interactions. By contrast, in the case of TEM-1 beta-lactamase mutations, aggregation is linked to a decreased cell fitness due to inactivation of protein function. Our study shows that aggregation is an important context-dependent constraint of molecular evolution and opens up new avenues to investigate the role of aggregation in the cell.
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spelling pubmed-82495732021-07-03 Aggregation is a Context-Dependent Constraint on Protein Evolution Monti, Michele Armaos, Alexandros Fantini, Marco Pastore, Annalisa Tartaglia, Gian Gaetano Front Mol Biosci Molecular Biosciences Solubility is a requirement for many cellular processes. Loss of solubility and aggregation can lead to the partial or complete abrogation of protein function. Thus, understanding the relationship between protein evolution and aggregation is an important goal. Here, we analysed two deep mutational scanning experiments to investigate the role of protein aggregation in molecular evolution. In one data set, mutants of a protein involved in RNA biogenesis and processing, human TAR DNA binding protein 43 (TDP-43), were expressed in S. cerevisiae. In the other data set, mutants of a bacterial enzyme that controls resistance to penicillins and cephalosporins, TEM-1 beta-lactamase, were expressed in E. coli under the selective pressure of an antibiotic treatment. We found that aggregation differentiates the effects of mutations in the two different cellular contexts. Specifically, aggregation was found to be associated with increased cell fitness in the case of TDP-43 mutations, as it protects the host from aberrant interactions. By contrast, in the case of TEM-1 beta-lactamase mutations, aggregation is linked to a decreased cell fitness due to inactivation of protein function. Our study shows that aggregation is an important context-dependent constraint of molecular evolution and opens up new avenues to investigate the role of aggregation in the cell. Frontiers Media S.A. 2021-06-18 /pmc/articles/PMC8249573/ /pubmed/34222334 http://dx.doi.org/10.3389/fmolb.2021.678115 Text en Copyright © 2021 Monti, Armaos, Fantini, Pastore and Tartaglia. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Monti, Michele
Armaos, Alexandros
Fantini, Marco
Pastore, Annalisa
Tartaglia, Gian Gaetano
Aggregation is a Context-Dependent Constraint on Protein Evolution
title Aggregation is a Context-Dependent Constraint on Protein Evolution
title_full Aggregation is a Context-Dependent Constraint on Protein Evolution
title_fullStr Aggregation is a Context-Dependent Constraint on Protein Evolution
title_full_unstemmed Aggregation is a Context-Dependent Constraint on Protein Evolution
title_short Aggregation is a Context-Dependent Constraint on Protein Evolution
title_sort aggregation is a context-dependent constraint on protein evolution
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8249573/
https://www.ncbi.nlm.nih.gov/pubmed/34222334
http://dx.doi.org/10.3389/fmolb.2021.678115
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