Paramagnetic Solid‐State NMR to Localize the Metal‐Ion Cofactor in an Oligomeric DnaB Helicase

Paramagnetic metal ions can be inserted into ATP‐fueled motor proteins by exchanging the diamagnetic Mg(2+) cofactor with Mn(2+) or Co(2+). Then, paramagnetic relaxation enhancement (PRE) or pseudo‐contact shifts (PCSs) can be measured to report on the localization of the metal ion within the protei...

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Detalles Bibliográficos
Autores principales: Zehnder, Johannes, Cadalbert, Riccardo, Terradot, Laurent, Ernst, Matthias, Böckmann, Anja, Güntert, Peter, Meier, Beat H., Wiegand, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Full Papers
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252064/
https://www.ncbi.nlm.nih.gov/pubmed/33822417
http://dx.doi.org/10.1002/chem.202100462

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8252064/
https://www.ncbi.nlm.nih.gov/pubmed/33822417
http://dx.doi.org/10.1002/chem.202100462

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