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Evaluation of alternative RNA extraction methods for detection of SARS-CoV-2 in nasopharyngeal samples using the recommended CDC primer-probe set

BACKGROUND: The efficiency of isolation and purification of the viral genome is a critical step to the accuracy and reliability of RT-qPCR to detect SARS-CoV-2. However, COVID-19 testing laboratories were overwhelmed by a surge in diagnostic demand that affected supply chains especially in low and m...

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Detalles Bibliográficos
Autores principales: Perez, Vinícius Pietta, Pessoa, Wallace Felipe Blohem, Galvão, Bruno Henrique Andrade, Sousa, Eduardo Sergio Soares, Dejani, Naiara Naiana, Campana, Eloiza Helena, Cavalcanti, Marilia Gabriela dos Santos, Cantarelli, Vlademir Vicente
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8253666/
https://www.ncbi.nlm.nih.gov/pubmed/35262017
http://dx.doi.org/10.1016/j.jcvp.2021.100032