High-content imaging and analysis to quantify the nuclear to cytoplasmic ratio of TGFβ and hippo effectors in mammalian cells

Automated high-content immunofluorescence (IF) microscopy is used to monitor and quantify localization of the TGFβ/Smads and Taz/Yap Hippo effectors in mouse epithelial EpH4 cells transfected with Taz/Yap siRNAs. The nuclear-to-cytoplasmic protein ratios obtained by IF are converted into normalized...

Descripción completa

Detalles Bibliográficos
Autores principales: Labibi, Bita, Bashkurov, Mikhail, Christova, Tania, Attisano, Liliana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254078/
https://www.ncbi.nlm.nih.gov/pubmed/34258593
http://dx.doi.org/10.1016/j.xpro.2021.100632

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8254078/
https://www.ncbi.nlm.nih.gov/pubmed/34258593
http://dx.doi.org/10.1016/j.xpro.2021.100632

Ejemplares similares