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Protocol for using TRIBE to study RNA-protein interactions and nuclear organization in mammalian cells

Targets of RNA-binding proteins discovered by editing (TRIBE) determines RNA-proteins interactions and nuclear organization with minimal false positives. We detail necessary steps for performing mammalian cell RBP-TRIBE to determine the targets of RNA-binding proteins and MS2-TRIBE to determine RNA-...

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Detalles Bibliográficos
Autores principales: Biswas, Jeetayu, Rosbash, Michael, Singer, Robert H., Rahman, Reazur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8255943/
https://www.ncbi.nlm.nih.gov/pubmed/34258595
http://dx.doi.org/10.1016/j.xpro.2021.100634