A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Characterizing protein–protein interactions (PPIs) is an effective method to help explore protein function. Here, through integrating a newly identified split human Rhinovirus 3 C (HRV 3 C) protease, super-folder GFP (sfGFP), and ClpXP-SsrA protein degradation machinery, we developed a fluorescence-...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Shengchen, Zhang, Faying, Mei, Meng, Wang, Ting, Yun, Yueli, Yang, Shihui, Zhang, Guimin, Yi, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260793/
https://www.ncbi.nlm.nih.gov/pubmed/34230602
http://dx.doi.org/10.1038/s42003-021-02374-w

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260793/
https://www.ncbi.nlm.nih.gov/pubmed/34230602
http://dx.doi.org/10.1038/s42003-021-02374-w

Ejemplares similares