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A split protease-E. coli ClpXP system quantifies protein–protein interactions in Escherichia coli cells

Characterizing protein–protein interactions (PPIs) is an effective method to help explore protein function. Here, through integrating a newly identified split human Rhinovirus 3 C (HRV 3 C) protease, super-folder GFP (sfGFP), and ClpXP-SsrA protein degradation machinery, we developed a fluorescence-...

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Detalles Bibliográficos
Autores principales:	Wang, Shengchen, Zhang, Faying, Mei, Meng, Wang, Ting, Yun, Yueli, Yang, Shihui, Zhang, Guimin, Yi, Li
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260793/ https://www.ncbi.nlm.nih.gov/pubmed/34230602 http://dx.doi.org/10.1038/s42003-021-02374-w

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