Synthesis of libraries and multi-site mutagenesis using a PCR-derived, dU-containing template

Directed DNA libraries are useful because they focus genetic diversity in the most important regions within a sequence. Ideally, all sequences in such libraries should appear with the same frequency and there should be no significant background from the starting sequence. These properties maximize t...

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Detalles Bibliográficos
Autores principales: Meinke, Gretchen, Dalda, Nahide, Brigham, Benjamin S, Bohm, Andrew
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260824/
https://www.ncbi.nlm.nih.gov/pubmed/34239985
http://dx.doi.org/10.1093/synbio/ysaa030

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260824/
https://www.ncbi.nlm.nih.gov/pubmed/34239985
http://dx.doi.org/10.1093/synbio/ysaa030

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