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Synthesis of libraries and multi-site mutagenesis using a PCR-derived, dU-containing template
Directed DNA libraries are useful because they focus genetic diversity in the most important regions within a sequence. Ideally, all sequences in such libraries should appear with the same frequency and there should be no significant background from the starting sequence. These properties maximize t...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260824/ https://www.ncbi.nlm.nih.gov/pubmed/34239985 http://dx.doi.org/10.1093/synbio/ysaa030 |