Optimized protocol for immunophenotyping of melanoma and tumor-bearing skin from mouse

While isolating immune cells from spleens and lungs is routinely achieved using flow cytometry, it is challenging to isolate viable immune cells from skin. Here, we describe a step-by-step protocol for skin digestion using a murine melanoma model, which is amenable for detection of low abundant immu...

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Detalles Bibliográficos
Autores principales: Krisna, Sai Sakktee, Goncalves, Christophe, Gagnon, Natascha, Huang, Fan, Plourde, Dany, Miller, Wilson H., Fritz, Jörg H., del Rincon, Sonia V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260876/
https://www.ncbi.nlm.nih.gov/pubmed/34258591
http://dx.doi.org/10.1016/j.xpro.2021.100627

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8260876/
https://www.ncbi.nlm.nih.gov/pubmed/34258591
http://dx.doi.org/10.1016/j.xpro.2021.100627

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