Protocol for nuclear export signal characterization of cGAS in mammalian cells

The cyclic GMP-AMP synthase (cGAS) is the principal DNA sensor, which binds DNA and triggers the type I interferon production. We used ISD45 or inactivated Vaccinia Virus (VACV) to stimulate cGAS and monitored cellular localization by immunofluorescence microscopy, Operetta high-content screening, a...

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Detalles Bibliográficos
Autores principales: Huang, Yu, McLean, Myles, Liang, Chen, Guo, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261000/
https://www.ncbi.nlm.nih.gov/pubmed/34278335
http://dx.doi.org/10.1016/j.xpro.2021.100649

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261000/
https://www.ncbi.nlm.nih.gov/pubmed/34278335
http://dx.doi.org/10.1016/j.xpro.2021.100649

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