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Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction
Single-molecule Förster resonance energy transfer (smFRET) is a powerful tool for investigating the dynamic properties of biomacromolecules. However, the success of protein smFRET relies on the precise and efficient labeling of two or more fluorophores on the protein of interest (POI), which has rem...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261736/ https://www.ncbi.nlm.nih.gov/pubmed/34276941 http://dx.doi.org/10.1039/d1sc02653d |