Single-molecule FRET and conformational analysis of beta-arrestin-1 through genetic code expansion and a Se-click reaction

Single-molecule Förster resonance energy transfer (smFRET) is a powerful tool for investigating the dynamic properties of biomacromolecules. However, the success of protein smFRET relies on the precise and efficient labeling of two or more fluorophores on the protein of interest (POI), which has rem...

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Detalles Bibliográficos
Autores principales: Han, Ming-Jie, He, Qing-tao, Yang, Mengyi, Chen, Chao, Yao, Yirong, Liu, Xiaohong, Wang, Yuchuan, Zhu, Zhong-liang, Zhu, Kong-kai, Qu, Changxiu, Yang, Fan, Hu, Cheng, Guo, Xuzhen, Zhang, Dawei, Chen, Chunlai, Sun, Jin-peng, Wang, Jiangyun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8261736/
https://www.ncbi.nlm.nih.gov/pubmed/34276941
http://dx.doi.org/10.1039/d1sc02653d

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